Genetic characterization of two low pathogenic avian influenza virus H5N1 isolates from Ontario, Canada
Identifieur interne : 001326 ( Main/Exploration ); précédent : 001325; suivant : 001327Genetic characterization of two low pathogenic avian influenza virus H5N1 isolates from Ontario, Canada
Auteurs : Yanlong Pei [Canada] ; Janet Swinton [Canada] ; Davor Ojkic [Canada] ; Shayan Sharif [Canada]Source :
- Virus Genes [ 0920-8569 ] ; 2009-02-01.
Descripteurs français
- Wicri :
- geographic : Canada.
English descriptors
- KwdEn :
Abstract
Abstract: Genomes of two low pathogenic H5N1 avian influenza (LPAI) viruses, A/Turkey/ON/84/1983 and A/Mallard/ON/499/2005 from Ontario, Canada were cloned and genetically characterized. Phylogenetic analysis showed that the Canadian isolates cluster with other North American AIVs and are distinct from the Euro-Asian H5N1 isolates. Individual gene comparisons demonstrated that the Ontario isolates were most similar to the viruses isolated from around the same time period and geographical area. A long deletion of 22 amino acids was identified in the stalk region of NA of A/Turkey/ON/84/1983 isolate, a characteristic mutation related to its adaptation to domestic birds. To our knowledge A/Turkey/ON/84/1983 genomic sequence is the first and only available entire genomic sequence of a H5N1 AIV from domestic birds in Canada and USA.
Url:
DOI: 10.1007/s11262-008-0311-4
Affiliations:
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Le document en format XML
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<front><div type="abstract" xml:lang="en">Abstract: Genomes of two low pathogenic H5N1 avian influenza (LPAI) viruses, A/Turkey/ON/84/1983 and A/Mallard/ON/499/2005 from Ontario, Canada were cloned and genetically characterized. Phylogenetic analysis showed that the Canadian isolates cluster with other North American AIVs and are distinct from the Euro-Asian H5N1 isolates. Individual gene comparisons demonstrated that the Ontario isolates were most similar to the viruses isolated from around the same time period and geographical area. A long deletion of 22 amino acids was identified in the stalk region of NA of A/Turkey/ON/84/1983 isolate, a characteristic mutation related to its adaptation to domestic birds. To our knowledge A/Turkey/ON/84/1983 genomic sequence is the first and only available entire genomic sequence of a H5N1 AIV from domestic birds in Canada and USA.</div>
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